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Animal models for scaffold implantationAnimals were housed in an AAALAC qualified facility,And all animal studies were performed under the supervision of Beth Israel Deaconess Medical Center Institutional Animal Care and Use Committee IACUC as per approved institutional protocol 155 2008. NCRNU nude mice 20 30g,6 8 weeks were paid for from Taconic Farms Germantown,new york. Prior to implant scaffolds,Mice were anesthetized with an intraperitoneal procedure of ketamine 100mg kg and xylazine 10mg kg.